CD4 Monoclonal Antibody (RM4-5), Pacific Orange,

货号：79-0042-80,79-0042-82

规格：25 ug ,100 μg

价格：1474,3607

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：RM4-5

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.Applications Reported: This RM4-5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RM4-5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Pacific Orange emits at 551 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 405 nm; Emission: 551 nm; Laser: Violet Laser.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

数据

CD4 Antibody (79-0042-80) in FlowC57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, eFluor 450 (Product # 48-0031-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, Pacific Orange (Product # 79-4321-81) (left) or 0.125 µg of CD4 Monoclonal Antibody, Pacific Orange (right). Cells in the lymphocyte gate were used for analysis.

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