CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), Biotin,

货号：13-0903-82,13-0903-85

规格：100 ug ,500 ug

价格：822,1959

产品类型：流式抗体

品牌：eBioscience

抗原：CD90.2 (Thy-1.2)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：30-H12

荧光染料：Biotin

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin,
浓度：	0.5 mg/mL	用法：	0.125 µg/test(Flow)

产品详细信息Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

数据

CD90.2 (Thy-1.2) Antibody (13-0903-82) in FlowStaining of BALB/c splenocytes with 0.06 µg of Rat IgG2b K Isotype Control Biotin (Product # 13-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD90-2 (Thy-1-2) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis. CD90.2 (Thy-1.2) Antibody (13-0903-82)Nature communications 2018 -Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 13-0903-82)

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参考文献

1. Nature communicationsA quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.2. Nature communicationsThe transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction.3. Nature biotechnologyNon-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin.4. Bio-protocolIn vitroDifferentiation of Murine Innate Lymphoid Cells from Common Lymphoid Progenitor Cells.5. Journal of cellular and molecular medicineHaematopoietic stem cells in spleen have distinct differentiative potential for antigen presenting cells.

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