CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), Functional Grade,
货号：16-0903-85,16-0903-38,16-0903-025 ,16-0903-050
规格：500 ug ,5 mg ,25 mg ,50 mg
价格：3624,24089,50625,120443
产品类型：流式抗体
品牌：eBioscience
抗原：CD90.2 (Thy-1.2)
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：30-H12
荧光染料：其它
| 类型: | 单抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade |
| 浓度： | 1 mg/mL | 用法： | 0.125 µg/test(Flow)；Assay-Dependent(FN) |
产品详细信息Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vivo and in vitro depletion.Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
| 数据 |
| CD90.2 (Thy-1.2) Antibody (16-0903-85) in FlowSurface staining of mouse splenocytes with Anti-Mouse CD90-2 (Thy-1-2) PE.Autofluorescence is shown via open histogram.Total viable cells were used for analysis. CD90.2 (Thy-1.2) Antibody (16-0903-85)Nature communications 2018 -Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 16-0903-85) |
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| 参考文献 |
| 1. Nature communicationsA quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.2. Nature communicationsWASH maintains NKp46+ILC3 cells by promoting AHR expression.3. PLoS pathogensLow doses of imatinib induce myelopoiesis and enhance host anti-microbial immunity.4. Nature immunologyCostimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.5. Mucosal immunologyOpposing consequences of IL-23 signaling mediated by innate and adaptive cells in chemically induced colitis in mice. |
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