CD69 Monoclonal Antibody (H1.2F3), eFluor 450, eBioscience™

货号：48-0691-82,48-0691-80

规格：100 µg,25 µg

价格：2278,1038

产品类型：流式抗体

品牌：eBioscience

抗原：CD69

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：H1.2F3

荧光染料：eFluor 450

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor 450, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of 24 hour Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.仅用于科研。不用于诊断过程。

数据

CD69 Antibody (48-0691-82) in FlowStaining of unstimulated (left) and onvernight ConA-stimulated (right) C57BL/6 splenocytes with 0.125 µg of Armenian Hamster IgG Isotype Control eFluor® 450 (Product # 48-4888-82) (blue histogram) or 0.125 µg of Anti-Mouse CD69 eFluor® 450 (purple histogram). Total viable cells were used for analysis.

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参考文献：

1. Bio-protocolEvaluation of Cross-presentation in Bone Marrow-derived Dendritic Cellsin vitroand Splenic Dendritic Cellsex vivoUsing Antigen-coated Beads."48-0691 was used in Flow cytometry/Cell sorting to develop a protocol for evaluating antigen cross-presentation by dendritic cells, both in vivo and ex vivo."AuthorsAlloatti A,Kotsias F,Hoffmann E,Amigorena SYear2016SpeciesMouse2. LeukemiaBTK inhibition results in impaired CXCR4 chemokine receptor surface expression, signaling and function in chronic lymphocytic leukemia."48-0691 was used in Flow cytometry/Cell sorting to identify CXCR4 as a key regulator in BTK-mediated CLL-cell retention."AuthorsChen SS,Chang BY,Chang S,Tong T,Ham S,Sherry B,Burger JA,Rai KR,Chiorazzi NYear2016SpeciesMouse3. Frontiers in immunologyQuantitative and Qualitative Analysis of Bone Marrow CD8(+) T Cells from Different Bones Uncovers a Major Contribution of the Bone Marrow in the Vertebrae."48-0691 was used in Flow cytometry/Cell sorting to suggest that in respect to CD8(+) T cells, BM harvested from a single bone is a fair reflection of the rest of the BM present in the murine body."AuthorsGeerman S,Hickson S,Brasser G,Pascutti MF,Nolte MA4. Nature protocolsIntravascular staining for discrimination of vascular and tissue leukocytes."48-0691 was used in Flow cytometry/Cell sorting to outline a protocol for the validation and use of intravascular staining to define innate and adaptive immune cells in mice."AuthorsAnderson KG,Mayer-Barber K,Sung H,Beura L,James BR,Taylor JJ,Qunaj L,Griffith TS,Vezys V,Barber DL,Masopust DYear2014SpeciesMouse5. NatureThemis sets the signal threshold for positive and negative selection in T-cell development."48-0691 was used in Flow cytometry/Cell sorting to show that the T-cell-specific protein Themis can specifically attenuate TCR signal strength via SHP1 recruitment and activation in response to low-affinity TCR engagement."AuthorsFu G,Casas J,Rigaud S,Rybakin V,Lambolez F,Brzostek J,Hoerter JA,Paster W,Acuto O,Cheroutre H,Sauer K,Gascoigne NR

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