CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Biotin,
货号：13-0902-82,13-0902-85
规格：100 ug ,500 ug
价格：822,1959
产品类型：流式抗体
品牌：eBioscience
抗原：CD90.2 (Thy-1.2)
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：53-2.1
荧光染料：Biotin
| 类型: | 单抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), Biotin |
| 浓度： | 0.5 mg/mL | 用法： | 0.06 µg/test(Flow) |
产品详细信息Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: The 53-2.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
| 数据 |
| CD90.2 (Thy-1.2) Antibody (13-0902-82) in FlowStaining of mouse splenocytes with Anti-Mouse CD90.2 (Thy-1.2) FITC (left) or PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. CD90.2 (Thy-1.2) Antibody (13-0902-82)Nature 2017 -Figure 2 Heterogeneity of CF and stepwise suppression of non-cardiomyocyte lineages during iCM induction ( a-b ) HC (a) and PCA (b) of control CFs with representative gene expression and GO analysis of the five identified gene clusters. ( c ) HC calculated with control CFs (a) applied to M+G+T-transduced cells with representative gene expression. ( d-f ) 40x ICC images (d,e) with quantifications (f) of Thy1 and SM22alpha co-stained with alphaMHC-GFP during reprogramming. n=20 images, scale bar=100 mum, error bars indicate SEM. |
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| 参考文献 |
| 1. NatureSingle-cell transcriptomics reconstructs fate conversion from fibroblast to cardiomyocyte.2. The Journal of clinical investigationMutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis.3. Annals of surgeryLymphatic Contribution to the Cellular Niche in Heterotopic Ossification.4. Nature communicationsDisruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.5. The Journal of clinical investigationThe composition of the microbiota modulates allograft rejection. |
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