CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine5,

货号：15-0902-82

规格：100 ug

价格：2677

产品类型：流式抗体

品牌：eBioscience

抗原：CD90.2 (Thy-1.2)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：53-2.1

荧光染料：PE-Cy5

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5
浓度：	0.2 mg/mL	用法：	0.06 µg/test(Flow)

产品详细信息Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: The 53-2.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

数据

CD90.2 (Thy-1.2) Antibody (15-0902-82) in FlowStaining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.03 µg Anti-Mouse CD90-2 (Thy-1-2) PE-Cyanine5 (filled histogram).Total cells were used for analysis. CD90.2 (Thy-1.2) Antibody (15-0902-82)The Journal of clinical investigation 2017 -Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82)

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参考文献

1. Journal of immunology (Baltimore, Md. : 1950)Enhancing T lineage production in aged mice: a novel function of Foxn1 in the bone marrow niche.2. The Journal of clinical investigationMutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis.3. Nature communicationsDisruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.4. Circulation researchCardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene.5. Nature communicationsDisruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.

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