CD8a Monoclonal Antibody (4SM15)
货号：14-0808-80,14-0808-82,14-0808-37,14-0808-95
规格：25μg,100μg,2mg,5mg
价格：1307,3268,11562,28906
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：其它
| 抗体类型 | 一抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 0.25 μg/test (Flow)； |
| 产品详细信息Description: The 4SM15 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 bindsto MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Recent studies have shown the 4SM15 antibody recognizes and binds a non-CD8a epitope in neural tissue. The 4SM15 clone is not recommended for staining of mouse neural tissue. For additional information please contact our technical service (tech@eBioscience.com). For detection of mouse CD8a in FFPE mouse neural tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no 14-0195).The 4SM15 monoclonal antibody is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD8a using immunohistochemistry of frozen tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no. 14-0081).Applications Reported: This 4SM15 antibody has been reported for use in flow cytometric analysis, microscopy, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 4SM15 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. The 4SM15 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
| CD8a Antibody (14-0808-80) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded mouse spleen using 5 µg/mL Anti-Mouse CD8a Purified, followed by Anti-Rat IgG Biotin, Streptavidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin. |
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| 参考文献： |
| 1.Nature communicationsNano-enabled pancreas cancer immunotherapy using immunogenic cell death and reversing immunosuppression."14-0808 was used in Immunohistochemistry to investigate how significant tumour reduction or eradication is accomplishable by recruiting cytotoxic T lymphocytes, concomitant with down-regulation of Foxp3+ T cells."2.Scientific reportsNiraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models."14-0808 was used in Immunohistochemistry on paraffin embedded tissues to investigate the therapeutic potential of combining niraparib with anti-P-1 immune checkpoint inhibitors in preclinical tumour models."3.Advanced materials (Deerfield Beach, Fla.)A Dual Immunotherapy Nanoparticle Improves T-Cell Activation and Cancer Immunotherapy."14-0808 was used in Immunohistochemistry on paraffin embedded tissues-immunofluorescence to demonstrate a novel strategy to improve combination immunotherapy using nanotechnology."4.Journal of translational medicineMicrowave ablation combined with OK-432 induces Th1-type response and specific antitumor immunity in a murine model of breast cancer.5.Journal of clinical & cellular immunologyDevelopment of inCVAX,In situCancer Vaccine, and Its Immune Response in Mice with Hepatocellular Cancer."14-0808 was used in Immunohistochemistry on paraffin embedded tissues to investigate the effect of inCVAX on immune activation in a murine model of hepatocellular cancer." |
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