CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1),

货号：14-0902-82,14-0902-85

规格：100 ug ,500 ug

价格：1394,1865

产品类型：流式抗体

品牌：eBioscience

抗原：CD90.2 (Thy-1.2)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：53-2.1

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.06 µg/test(Flow)；Assay-Dependent(IP)

产品详细信息Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.Applications Reported: The 53-2.1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.Applications Tested: The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

数据

CD90.2 (Thy-1.2) Antibody (14-0902-82) in FlowSurface staining of mouse splenocytes with Anti-Mouse CD90-2 (Thy-1-2) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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参考文献

1. ImmunityRetinoic Acid Differentially Regulates the Migration of Innate Lymphoid Cell Subsets to the Gut.2. Cell death & diseaseRenin-angiotensin system regulates neurodegeneration in a mouse model of normal tension glaucoma.3. Experimental neurologyThe generation of olfactory epithelial neurospheres in vitro predicts engraftment capacity following transplantation in vivo.4. NatureSingle-cell transcriptomics reconstructs fate conversion from fibroblast to cardiomyocyte.5. Nature communicationsNetrin-1 regulates somatic cell reprogramming and pluripotency maintenance.

技术参数

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