CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), eFluor 450,

货号：48-0900-82

规格：100 µg

价格：2431

产品类型：流式抗体

品牌：eBioscience

抗原：CD90.1 (Thy-1.1)

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：HIS51

荧光染料：eFluor 450

类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), eFluor 450
浓度：	0.2 mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

数据

CD90.1 (Thy-1.1) Antibody (48-0900-82) in FlowStaining of rat splenocytes with Anti-Rat CD3 FITC (Product # 11-0030-82) and 0.125 µg of Mouse IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4724-82) (left) or 0.125 µg of Anti-Mouse/Rat CD90.1 (Thy-1.1) eFluor® 450 (right). Total viable cells were used for analysis.

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参考文献

1. Clinical cancer research : an official journal of the American Association for Cancer ResearchHeterodimeric IL15 Treatment Enhances Tumor Infiltration, Persistence, and Effector Functions of Adoptively Transferred Tumor-specific T Cells in the Absence of Lymphodepletion.2. Frontiers in endocrinologyAldosterone Blocks Rat Stem Leydig Cell DevelopmentIn Vitro.3. Nature communicationsIL-21 restricts T follicular regulatory T cell proliferation through Bcl-6 mediated inhibition of responsiveness to IL-2.4. Frontiers in endocrinologyAldosterone Blocks Rat Stem Leydig Cell DevelopmentIn Vitro.5. NatureSingle-cell transcriptomics reconstructs fate conversion from fibroblast to cardiomyocyte.

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