CD69 Monoclonal Antibody (H1.2F3), PE-Cyanine5, eBioscience™

货号：15-0691-82

规格：100 µg

价格：2840

产品类型：流式抗体

品牌：eBioscience

抗原：CD69

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

荧光染料：PE-Cy5

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5, eBioscience™
浓度：	0.2 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: The H1.2F3antibody has been reported for use in flow cytometric analysis.Applications Tested: The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.仅用于科研。不用于诊断过程。

数据

CD69 Antibody (15-0691-82) in FlowStaining of non-stimulated (left) and 5-hour PMA activated C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg Anti-Mouse CD69 PE-Cyanine5 (filled histogram).Total cells were used for analysis.

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参考文献：

1.EndocrinologyIRF6 Regulates Alternative Activation by Suppressing PPARγ in Male Murine Macrophages."Published figure using CD69 monoclonal antibody (Product # 15-0691-82) in Flow Cytometry"2.Cell death & diseaseCD69 enhances immunosuppressive function of regulatory T-cells and attenuates colitis by prompting IL-10 production."15-0691 was used in Flow cytometry/Cell sorting to study the importance of the C-type lectin receptor CD69 to the suppressive function of Tregs."AuthorsYu L,Yang F,Zhang F,Guo D,Li L,Wang X,Liang T,Wang J,Cai Z,Jin H3.The Journal of clinical investigationMicroRNA-223 is a crucial mediator of PPARγ-regulated alternative macrophage activation."Published figure using CD69 monoclonal antibody (Product # 15-0691-82) in Flow Cytometry"4.Infection and immunityModulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection."Published figure using CD69 monoclonal antibody (Product # 15-0691-82) in Flow Cytometry"5.Infection and immunityMycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy."15-0691 was used in Flow cytometry/Cell sorting to study how the interplay between M. tuberculosis and NK cells/APC triggering IFN-gamma may play a beneficial role in tuberculous pleurisy by helping to maintain a type 1 profile."AuthorsSchierloh P,Yokobori N,Alemán M,Landoni V,Geffner L,Musella RM,Castagnino J,Baldini M,Abbate E,de la Barrera SS,Sasiain MC

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