CD69 Monoclonal Antibody (H1.2F3), PE, eBioscience™

货号：12-0691-81,12-0691-82,12-0691-83

规格：50 µg,100 µg,200 µg

价格：1164,2081,2840

产品类型：流式抗体

品牌：eBioscience

抗原：CD69

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：H1.2F3

荧光染料：PE

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: The H1.2F3antibody has been reported for use in flow cytometric analysis.Applications Tested: The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.仅用于科研。不用于诊断过程。

数据

CD69 Antibody (12-0691-81) in FlowStaining of unstimulated (left) or overnight ConA-stimulated (right) C57BL/6 mouse splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 0.25 µg of Anti-Mouse CD69 PE (purple histogram). Total viable cells were analyzed.

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参考文献：

1.Molecular and cellular biologyIncreased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection."12-0691 was used in Western Blotting to determine the effects of increased p300 expression on GR-TCR crosstalk between thymocytes."AuthorsYu CT,Feng MH,Shih HM,Lai MZ2.OncoimmunologyLYG1 exerts antitumor function through promoting the activation, proliferation, and function of CD4+T cells."12-0691 was used in Flow cytometry/Cell sorting to reveal a tumor-inhibiting role for LYG1 through promoting the activation, proliferation, and function of CD4+T cells in antitumor immune responses, offering implications for novel tumor immunotherapy."AuthorsLiu H,Zhang Y,Liu Z,Wang P,Mo X,Fu W,Liu W,Cheng Y,Han W3.NPJ vaccinesEnhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules."Published figure using CD69 monoclonal antibody (Product # 12-0691-82) in Flow Cytometry"4.Protein & cellContact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival."Published figure using CD69 monoclonal antibody (Product # 12-0691-82) in Flow Cytometry"5.BMC infectious diseasesA peptide fragment from the human COX3 protein disrupts association of Mycobacterium tuberculosis virulence proteins ESAT-6 and CFP10, inhibits mycobacterial growth and mounts protective immune response."12-0691 was used in in vitro experiments to show that disruption of ESAT-6:CFP10 association could be an alternate method for attenuating M. tuberculosis."AuthorsSamuchiwal SK,Tousif S,Singh DK,Kumar A,Ghosh A,Bhalla K,Prakash P,Kumar S,Bhattacharyya M,Moodley P,Das G,Ranganathan A

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