FOXP3 Monoclonal Antibody (FJK-16s), FITC,

货号：11-5773-80,11-5773-82

规格：25μg,100μg

价格：2534,3783

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：FJK-16s

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC
浓度：	0.5 mg/mL	用法：	1 µg/test(Flow)；Assay-Dependent(IHC (F))

产品详细信息Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (cat. 00-5523). The use of other fixation and staining buffers is not recommended.Applications Reported: This FJK-16s antibody has been reported for use in intracellular flow cytometric analysis. For optimal staining with FJK-16s, it is highly recommended to use the Anti-Mouse/Rat Foxp3 FITC Staining Set (cat. 71-5775). FJK-16s has also been reported for use in immunohistochemical staining of frozen tissue sections.Applications Tested: This FJK-16s antibody has been tested by intracellular flow cytometric analysis of mouse splenocytes using the Anti-Mouse Foxp3 FITC Staining Set (cat. 71-5775) and protocol. Please refer to intracellular Staining Protocol on Best Protocol web page - Protocol B: One-step protocol for intracellular (nuclear) proteins. This antibody can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

数据

FOXP3 Antibody (11-5773-80) in FlowSurface staining of BALB/c splenocytes with Anti-Mouse CD4 PE (Product # 12-0042-82) and Anti-Mouse CD25 APC (Product # 17-0251-82), and subsequently fixed and permeabilized using the Foxp3 Staining Buffers (Product # 00-5523-00) and stained with 1.0 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) or 1.0 µg of Anti-Mouse/Rat Foxp3 FITC. The histogram (left) demonstrates Foxp3 staining (filled histogram) or isotype control staining (open histogram) after gating on CD4+CD25+ cells. The dot plot (right) demonstrates co-staining of CD4 and Foxp3. Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Molecular therapy : the journal of the American Society of Gene TherapyGenetic-induced variations in the GAD65 T-cell repertoire governs efficacy of anti-CD3/GAD65 combination therapy in new-onset type 1 diabetes.2. Cancer immunology researchDecitabine Enhances Lymphocyte Migration and Function and Synergizes with CTLA-4 Blockade in a Murine Ovarian Cancer Model.3. The American journal of pathologyFoxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.4. DiabetesDietary supplementation with high doses of regular vitamin D3 safely reduces diabetes incidence in NOD mice when given early and long term.5. Journal of neuroimmunologyHeme oxygenase-1 protects regulatory T cells from hypoxia-induced cellular stress in an experimental mouse brain tumor model.

技术参数

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