Anti-Mouse/Rat Foxp3 Staining Set PE,
货号：72-5775-40
规格：1 kit
价格：4101
产品类型：流式抗体
品牌：eBioscience
抗原：Foxp3
物种：其它
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：FJK-16s
荧光染料：PE
| 类型: | 单抗 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | Flow |
The FJK-16s antibody reacts with mouse, rat, dog, porcine, and bovine Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3 a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.Intracellular staining of mouse splenocytes with FJK-16s using the PE anti-mouse/rat Foxp3 Staining Set and protocol reveals approximately 2% of total cells in the C57Bl/6 strain and approximately 3-5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+CD25+ cells and 4% of the CD4+CD25- cells staining with FJK-16s. B220+, CD11b+, CD11c+, and Ly6G/Gr-1+ cells do not show significant co-staining with FJK-16s.
| 数据 |
Data for Anti-Mouse/Rat Foxp3 Staining Set PEBALB/c splenocytes were surface stained with Anti-Mouse CD4 FITC (cat. 11-0042) and Anti-Mouse CD25 APC (cat. 17-0251) then intracellularly stained with 0.5 µg of Rat IgG2a K Isotype Control PE (cat. 12-4321) or 0.5 µg of Anti-Mouse/Rat Foxp3 PE using the Anti-Mouse/Rat Foxp3 Staining Set and protocol. Cells in the lymphocyte gate were used for analysis. |
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