CD8a Monoclonal Antibody (53-6.7), Super Bright 600
货号：63-0081-80,63-0081-82
规格：25μg,100μg
价格：1513,3634
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG1, kappa
荧光染料：Super Bright 600
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 600 |
| 浓度： | 0.2 mg/mL | 用法： | 0.5 μg/test (Flow)； |
| 产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. |
| 数据 |
| CD8a Antibody (63-0081-80) in FlowStaining of mouse splenocytes with Anti-Mouse CD3 APC (Product # 17-0032-82) and 0.25 µg of Rat IgG2a K Isotype Control Super Bright 600 (Product # 63-4321-82) (left) or 0.25 µg of Anti-Mouse CD8a Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Scientific reportsType 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis."Published figure using CD8a monoclonal antibody (Product # 63-0081-82) in Immunohistochemistry"2.OncotargetUse of tilapia piscidin 3 (TP3) to protect against MRSA infection in mice with skin injuries."Published figure using CD8a monoclonal antibody (Product # 63-0081-82) in Immunohistochemistry"3.Proceedings of the National Academy of Sciences of the United States of AmericaCD31 signals confer immune privilege to the vascular endothelium."Published figure using CD8a monoclonal antibody (Product # 63-0081-82) in Flow Cytometry"4.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsImaging Tolerance Induction in the Classic Medawar Neonatal Mouse Model: Active Roles of Multiple F1-Donor Cell Types."Published figure using CD8a monoclonal antibody (Product # 63-0081-82) in Immunocytochemistry"5.Nature communicationsOncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion."Published figure using CD8a monoclonal antibody (Product # 63-0081-82) in Immunofluorescence" |
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