CD28 Monoclonal Antibody (37.51), Biotin,

货号：13-0281-82,13-0281-85

规格：100 ug,500 ug

价格：1822,2629

产品类型：流式抗体

品牌：eBioscience

抗原：CD28

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：37.51

荧光染料：Biotin

类型:	单抗	同型对照：	Syrian Hamster IgG Isotype Control, Biotin
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

数据

CD28 Antibody (13-0281-82) in FlowStaining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.25 µg of Golden Syrian Hamster IgG Isotype Control Biotin (Product # 12-4914-81) (left) or 0.25 µg of Anti-Mouse CD28 Biotin (right) and then detected with Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献

1. Nature communicationsT cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells.2. BMC cancerOverexpression of IL-15 promotes tumor destruction via NK1.1+ cells in a spontaneous breast cancer model.3. eLifeDifferential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.4. Nature communicationsThe ER membrane-anchored ubiquitin ligase Hrd1 is a positive regulator of T-cell immunity.5. Shock (Augusta, Ga.)Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

技术参数

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