CD28 Monoclonal Antibody (37.51), APC,
货号：17-0281-82
规格：100 ug
价格：2840
产品类型：流式抗体
品牌：eBioscience
抗原：CD28
物种：小鼠
宿主：其它
抗体亚型：IgG
克隆号：37.51
荧光染料：APC
| 类型: | 单抗 | 同型对照： | Syrian Hamster IgG Isotype Control, APC |
| 浓度： | 0.2 mg/mL | 用法： | 0.5 µg/test(Flow) |
产品详细信息Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
| 数据 |
CD28 Antibody (17-0281-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.25 µg of Golden Syrian Hamster IgG Isotype Control APC (Product # 17-4914-81) (left) or 0.25 µg of Anti-Mouse CD28 APC (right). Total viable cells were used for analysis. |
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| 参考文献 |
| 1. Molecular cellCTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.2. The Journal of experimental medicineLight chain editing generates polyreactive antibodies in chronic graft-versus-host reaction.3. Nature communicationsThe ER membrane-anchored ubiquitin ligase Hrd1 is a positive regulator of T-cell immunity.4. Journal of immunology (Baltimore, Md. : 1950)Occupancy of lymphocyte LFA-1 by surface-immobilized ICAM-1 is critical for TCR- but not for chemokine-triggered LFA-1 conversion to an open headpiece high-affinity state.5. Shock (Augusta, Ga.)Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression. |
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