CD28 Monoclonal Antibody (37.51),

货号：14-0281-81,14-0281-82,14-0281-85,14-0281-86

规格：50μg ,100μg ,500μg ,1 mg

价格：582,1132,2511,4818

产品类型：流式抗体

品牌：eBioscience

抗原：CD28

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：37.51

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)；Assay-Dependent(IHC)；Assay-Dependent(IP)；Assay-Dependent(FN)

产品详细信息Description: The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The 37.51 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 37.51 has also been reported in costimulation of T cells in vitro and in vivo . (Please use Functional Grade purified 37.51, cat. 16-0281, in functional assays.).Applications Tested: The 37.51 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

数据

CD28 Antibody (14-0281-81) in FlowStaining of BALB/c thymocytes with 0.25 µg of Golden Syrian Hamster IgG Isotype Control Purified (Product # 14-4914-81) (open histogram) or 0.25 µg of Anti-Mouse CD28 Purified (filled histogram) followed by Anti-Syrian Hamster IgG FITC (Product # 11-4211-85). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Molecular medicine (Cambridge, Mass.)Role of glycine N-methyltransferase in the regulation of T-cell responses in experimental autoimmune encephalomyelitis.2. Nature communicationsNCoR1 restrains thymic negative selection by repressing Bim expression to spare thymocytes undergoing positive selection.3. Nature communicationsT cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells.4. Frontiers in immunologySirtuin 2 Deficiency Increases Bacterial Phagocytosis by Macrophages and Protects from Chronic Staphylococcal Infection.5. BMC cancerOverexpression of IL-15 promotes tumor destruction via NK1.1+ cells in a spontaneous breast cancer model.

技术参数

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