| 产品介绍 | | |
| Sheep anti Bovine IgG polyclonal antibodyrecognizes bovine IgG and shows no cross - reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. This polyclonal antibody has not been cross adsorbed and may therefore react with IgG from other speciesSheep anti Bovine IgG has been usefully employed for the detection of antigen specific antibody reactivity in cattle by ELISA (Vrielinget al.2013). |
| 产品详情 | | |
Target Species | Bovine |
Product Form | Purified IgG - liquid |
| Antiserum Preparation | Antisera to bovine IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
Immunogen | Purified bovine IgG. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Immunodiffusion | √ | | |
| ELISA | √ | 1/100 | 1/30000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Somda, M.B.et al.(2016) Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.Parasit Vectors. 9 (1): 149.2. Facciuolo, A.et al.(2016) Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer's Patches toMycobacterium aviumsubsp.paratuberculosisSecreted Proteins following Targeted Enteric Infection in Young Calves.PLoS One. 11 (7): e0158747.3. Subharat, S.et al.(2015) Vaccination of cattle with a methanogen protein produces specific antibodies in the saliva which are stable in the rumen.Vet Immunol Immunopathol. 164 (3-4): 201-7.4. Benedictus, L.et al.(2016) Pregnancy boosts vaccine-induced Bovine Neonatal Pancytopenia-associated alloantibodies.Vaccine. 34 (8): 1002-5.5. Denholm, S.J.et al.(2018) Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements.J Dairy Sci. 101 (11): 10248-10258.6. Alo, Ket al.(2018) Passive protective effect of anti-K99 antibodies against enterotoxigenicE.coli.infection in neonatal calvesIr J of Vet Med, 12(2), 97-1077. Somda, M.B.et al.(2013) First insights into the cattle serological response to tsetse salivary antigens: a promising direct biomarker of exposure to tsetse bites.Vet Parasitol. 197 (1-2): 332-40.8. Waap, H.et al.(2011) A modified agglutination test for the diagnosis ofBesnoitia besnoiti.infection.Vet Parasitol. 178 (3-4): 217-22.9. Springer, A.et al.(2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine LungwormDictyocaulus viviparus..Pathogens 2022, 11, 55.10. Anastácio, C.et al.(2022) Impact of Endemic Besnoitiosis on the Performance of a Dairy Cattle Herd.Animals (Basel). 12 (10): 1291. |
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