CD27 Monoclonal Antibody (LG.7F9), PerCP-eFluor 710, eBioscience™

货号：46-0271-82,46-0271-80

规格：100 µg,25µg

价格：3341,1132

产品类型：流式抗体

品牌：eBioscience

抗原：CD27

物种：人/小鼠/大鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：LG.7F9

荧光染料：PerCP-eFluor™ 710

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PerCP-eFluor 710, eBioscience™
浓度：	0.2 mg/mL	用法：	0.06 µg/test（Flow）

产品详细信息Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.Applications Reported: This LG.7F9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This LG.7F9 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.仅用于科研。不用于诊断过程。

数据

CD27 Antibody (46-0271-82) in FlowStaining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and staining buffer (autofluorescence) (left) or 0.03 µg of Anti-CD27 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Cell reportsQa-1-Restricted CD8+T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection."46-0271 was used in Flow cytometry/Cell sorting to study compensatory mechanisms in immune responses to viruses when classical T cell immunity has been evaded."AuthorsAnderson CK,Reilly EC,Lee AY,Brossay L2. European journal of immunologyActivated NKT cells imprint NK-cell differentiation, functionality and education."46-0271 was used in Flow cytometry/Cell sorting to study the cellular cross-talk between natural killer T cells and natural killer cells."AuthorsRiese P,Trittel S,May T,Cicin-Sain L,Chambers BJ,Guzmán CAYear2015SpeciesMouse 3. PloS oneRole of NK cell subsets in organ-specific murine melanoma metastasis."46-0271 was used in Flow cytometry/Cell sorting to examine the organ-specific functions of natural killer cells."AuthorsBallas ZK,Buchta CM,Rosean TR,Heusel JW,Shey MR 4. Nature communicationsNK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation."Published figure using CD27 monoclonal antibody (Product # 46-0271-82) in Flow Cytometry"5. Cellular & molecular immunologyThe breast tumor microenvironment alters the phenotype and function of natural killer cells."46-0271 was used in Flow cytometry/Cell sorting to investigate the developmental stage of tumour-associated NK cells, showing that the tumour microenvironment alters the phenotype and function of NK cells."

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