CD27 Monoclonal Antibody (LG.7F9), eBioscience™

货号：14-0271-85,14-0271-82

规格：500 µg,100 µg

价格：2872,1136

产品类型：流式抗体

品牌：eBioscience

抗原：CD27

物种：人/小鼠/大鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：LG.7F9

荧光染料：Purified

类型:	一抗	同型对照：	IgG
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. Use of mild detergent lysates is recommended when performing immunoprecipitation with this mAb. LG.7F9 has also been reported in blocking of ligand binding. (Please use Functional Grade purified LG.7F9, cat. 16-0271, in functional assays.).Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.仅用于科研。不用于诊断过程。

数据

CD27 Antibody (14-0271-85) in FlowStaining of mouse splenocytes with 0.25 µg Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg Anti-CD27 Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111). Total viable cells were used for analysis.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献：

1. Cell reportsNon-redundant ISGF3 Components Promote NK Cell Survival in an Auto-regulatory Manner during Viral Infection.AuthorsGeary CD,Krishna C,Lau CM,Adams NM,Gearty SV,Pritykin Y,Thomsen AR,Leslie CS,Sun JCYear2018SpeciesMouse 2. Nature communicationsTBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival."14-0271 was used in Flow cytometry/Cell sorting to study the mechanism of IL-15 function in autophagy and natural killer cell survival."AuthorsZhu L,Xie X,Zhang L,Wang H,Jie Z,Zhou X,Shi J,Zhao S,Zhang B,Cheng X,Sun SCYear2018SpeciesMouseDilution1:300 3. The Journal of experimental medicineContinuous activity of Foxo1 is required to prevent anergy and maintain the memory state of CD8+T cells."14-0271 was used in Flow cytometry/Cell sorting to show that FOXO1 is continuously required for all the phenotypic characteristics of memory-effector T cells."AuthorsDelpoux A,Michelini RH,Verma S,Lai CY,Omilusik KD,Utzschneider DT,Redwood AJ,Goldrath AW,Benedict CA,Hedrick SMYear2018SpeciesMouse 4. European journal of immunologyNCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation."14-0271 was used in Flow cytometry/Cell sorting to provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection."AuthorsMiletic A,Lenartic M,Popovic B,Brizic I,Trsan T,Miklic K,Mandelboim O,Krmpotic A,Jonjic SYear2017SpeciesMouse 5. LeukemiaCasein kinase 2 controls the survival of normal thymic and leukemic γδ T cells via promotion of AKT signaling."14027182 was used in flow cytometry to identify a role for CK2 in healthy human thymocytes in the selection of the gamma delta T-cell lineage"AuthorsRibeiro ST,Tesio M,Ribot JC,Macintyre E,Barata JT,Silva-Santos B

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-27623.htm