CD25 Monoclonal Antibody (eBio3C7 (3C7)), eFluor 450, eBioscience™

货号：48-0253-82

规格：100μg

价格：2323

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：eBio3C7 (3C7)

荧光染料：eFluor 450

抗体类型:	小鼠流式抗体	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor 450, eBioscience
浓度：	0.2 mg/mL	用法：	1 µg/test (Flow)

Description:The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody.Applications Reported: This eBio3C7 (3C7) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio3C7 (3C7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD25 Antibody (48-0253-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD4 APC (Product # 17-0041-82) and 0.5 µg of Rat IgG2b kappa Isotype Control eFluor® 450 (Product # 48-4031-82) (left) or 0.5 µg of Anti-Mouse CD25 eFluor® 450 (right). Total viable cells were used for analysis.

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参考文献：

1.Stem cell reportsMFG-E8 Is Critical for Embryonic Stem Cell-Mediated T Cell Immunomodulation."48-0253 was used in Flow cytometry/Cell sorting to elucidate the molecules and mechanisms pertinent to the low immunogenicity of undifferentiated embryonic stem cells."2.Respiratory researchHeterogeneity of respiratory dendritic cell subsets and lymphocyte populations in inbred mouse strains."Published figure using CD25 monoclonal antibody (Product # 48-0253-82) in Flow Cytometry"3.European journal of immunologyReduced TCR-dependent activation through citrullination of a T-cell epitope enhances Th17 development by disruption of the STAT3/5 balance."48-0253 was used in Flow cytometry/Cell sorting to investigate how citrullination of an immunedominant T-cell epitope affects Th17 development."4.Respiratory researchHeterogeneity of respiratory dendritic cell subsets and lymphocyte populations in inbred mouse strains."Published figure using CD25 monoclonal antibody (Product # 48-0253-82) in Flow Cytometry"

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