CD137 (4-1BB) Monoclonal Antibody (17B5), PE,

货号：12-1371-81,12-1371-82,12-1371-83

规格：50 ug ,100 ug ,200 ug

价格：1038,1984,3050

产品类型：流式抗体

品牌：eBioscience

抗原：CD137 (4-1BB)

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：17B5

荧光染料：PE

类型:	单抗	同型对照：	Syrian Hamster IgG Isotype Control, PE,
浓度：	0.2 mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily. CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex. Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction. Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation. The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays.Applications Reported: The 17B5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 17B5 antibody has been tested by flow cytometric analysis of activated mouse splenocytes cultures. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

数据

CD137 (4-1BB) Antibody (12-1371-81) in FlowStaining of 3-day unstimulated (left) and 3-day ConA-stimulated (right) BALB/c splenocytes with 0.125 µg of Golden Syrian Hamster IgG Isotype Control PE (Product # 12-4914-81) (open histogram) or 0.125 µg of Anti-Mouse CD137 (4-1BB) PE (filled histogram). Total viable cells were used for analysis.

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参考文献

1. Journal of neuroinflammationCD137 ligand activated microglia induces oligodendrocyte apoptosis via reactive oxygen species.2. PloS oneRegulation of granulocyte and macrophage populations of murine bone marrow cells by G-CSF and CD137 protein.3. European journal of microbiology & immunologyImpact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function.4. Journal of neuroinflammationCD137 ligand activated microglia induces oligodendrocyte apoptosis via reactive oxygen species.5. European journal of microbiology & immunologyImpact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function.

技术参数

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