CD137 (4-1BB) Monoclonal Antibody (17B5), PE-Cyanine7,

货号：25-1371-80,25-1371-82

规格：25 ug ,100 ug

价格：1267,3050

产品类型：流式抗体

品牌：eBioscience

抗原：CD137 (4-1BB)

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：(17B5)

荧光染料：PE-Cy7

类型:	单抗	同型对照：	Syrian Hamster IgG Isotype Control, PE-Cyanine7
浓度：	0.2 mg/mL	用法：	1 µg/test(Flow)

产品详细信息Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily. CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex. Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction. Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation. The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays.Applications Reported: This 17B5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 17B5 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

数据

CD137 (4-1BB) Antibody (25-1371-80) in FlowStaining of unstimulated (left) or 3-day Con A-stimulated (right) mouse splenocytes with Anti-Mouse CD278 (ICOS) PE (Product # 12-9942-82) and 0.5 µg of Anti-Mouse CD137 (4-1BB) PE-Cyanine7. Viable cells in the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.

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参考文献

1. Cell reportsPulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer.2. Cancer immunology researchVaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8+T Cells.3. Journal of immunology (Baltimore, Md. : 1950)The 4-1BB costimulation augments the proliferation of CD4+CD25+ regulatory T cells.

技术参数

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