| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.25 μg)/test (Flow) |
| 产品详细信息Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (15-0049-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 Isotype Control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or Anti-Human CD4 PE-Cyanine5 (filled histogram).Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Frontiers in immunologyThe Synergistic Effects of the Glutathione Precursor, NAC and First-Line Antibiotics in the Granulomatous Response AgainstMycobacterium tuberculosis."15-0049 was used in Immunocytochemistry-immunoflourescence to examine if N-Acetyl Cysteine in combination with antibiotics would be sufficient to completely clear Mycobacterium tuberculosis infection within in vitro granulomas."AuthorsTeskey G,Cao R,Islamoglu H,Medina A,Prasad C,Prasad R,Sathananthan A,Fraix M,Subbian S,Zhong L,Venketaraman V2.Frontiers in immunologyThe Synergistic Effects of the Glutathione Precursor, NAC and First-Line Antibiotics in the Granulomatous Response AgainstMycobacterium tuberculosis."15-0049 was used in Immunocytochemistry-immunoflourescence to examine if N-Acetyl Cysteine in combination with antibiotics would be sufficient to completely clear Mycobacterium tuberculosis infection within in vitro granulomas."AuthorsTeskey G,Cao R,Islamoglu H,Medina A,Prasad C,Prasad R,Sathananthan A,Fraix M,Subbian S,Zhong L,Venketaraman V3.Cancer immunology, immunotherapy : CIIIdentification of two HLA-A*0201 immunogenic epitopes of lactate dehydrogenase C (LDHC): potential novel targets for cancer immunotherapy."Published figure using CD4 monoclonal antibody (Product # 15-0049-42) in Flow Cytometry"4.Frontiers in immunologyImpact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis."Published figure using CD4 monoclonal antibody (Product # 15-0049-42) in Flow Cytometry"5.Molecular medicine reportsMicroRNA‑155 inhibits the proliferation of CD8+ Tcells via upregulating regulatory T cells in vitiligo."Published figure using CD4 monoclonal antibody (Product # 15-0049-42) in Flow Cytometry" |
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