CD20 Monoclonal Antibody (2H7), APC-eFluor 780, eBioscience

货号：47-0209-42

规格：100 Tests

价格：3097

产品类型：流式抗体

品牌：eBioscience

抗原：CD20

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：2H7

荧光染料：APC-eFluor 780

抗体类型:	人CD20细胞流式抗体	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), APC-eFluor 780, eBioscience
浓度：	5 μL/Test	用法：	5μL（0.25 µg）/test (Flow)

Description:The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.Applications Reported: This 2H7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blod cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD20 Antibody (47-0209-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) and Mouse IgG2b K Isotype Control APC-eFluor® 780 (Product # 47-4732-80) (left) or Anti-Human CD20 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of translational medicineAutomation of cellular therapy product manufacturing: results of a split validation comparing CD34 selection of peripheral blood stem cell apheresis product with a semi-manual vs. an automatic procedure."47-0209 was used in Flow cytometry/Cell sorting to test the feasiblity of CD34 cell selection using an immunomagnetic "normal scale" selection module on the cell processing automat CliniMACS Prodigy."2.Clinical cancer research : an official journal of the American Association for Cancer ResearchPhase I study of a bispecific ligand-directed toxin targeting CD22 and CD19 (DT2219) for refractory B-cell malignancies."Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry"3.The Journal of experimental medicinePeripherally derived T regulatory and γδ T cells have opposing roles in the pathogenesis of intractable pediatric epilepsy."47-0209 was used in Flow cytometry/Cell sorting to study the roles of peripherally derived regulatory and γΔ T cells in the pathogenesis of intractable paediatric epilepsy."4.Nature communicationsEvidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation."Published figure using CD20 monoclonal antibody (Product # 47-0209-42) in Flow Cytometry"

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