CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE-Cyanine5
货号：15-9942-82
规格：100μg
价格：2732
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
荧光染料：PE-Cy5
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine5 |
| 浓度： | 0.2 mg/mL | 用法： | 0.5 μg/test(Flow) |
| 产品详细信息Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis.Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. |
| 数据 |
| CD278 (ICOS) Antibody (15-9942-82) in FlowStaining of unstimulated (left) and 3-day Con A-stimulated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b K Isotype Control PE-Cyanine5 (Product # 15-4031-82) (blue histogram) or 0.06 µg of Anti-Mouse CD278 (ICOS) PE-Cyanine5 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 660, were used for analysis. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1.Nature communicationsPD-L1hi B cells are critical regulators of humoral immunity."15-9942 was used in Flow cytometry/Cell sorting to demonstrate that elevated PD-L1 expression on B cells is an important regulator of T follicular helper-cell activity, downstream antibody responses and autoimmune disease."AuthorsKhan AR,Hams E,Floudas A,Sparwasser T,Weaver CT,Fallon PG2.Nature communicationsTargeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment."Published figure using CD278 (ICOS) monoclonal antibody (Product # 15-9942-82) in Flow Cytometry"3.Journal of immunology (Baltimore, Md. : 1950)Blockade of B7-H1 (programmed death ligand 1) enhances humoral immunity by positively regulating the generation of T follicular helper cells."15-9942 was used in Flow cytometry/Cell sorting to demonstrate that the coinhibitory B7-H1/programmed death 1 pathway can limit the expansion of T(FH) cells and constrain Ag-specific Ig responses."4.Current protocols in mouse biologyA Mouse Model of West Nile Virus Infection."15-9942 was used in Flow cytometry/Cell sorting to develop a mouse model to study the breadth of symptoms and disease severity seen in human West Nile virus infection."5.Nature communicationsTargeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment."Published figure using CD278 (ICOS) monoclonal antibody (Product # 15-9942-82) in Flow Cytometry" |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-27713.htm
QQ:1749072012