CD20 Monoclonal Antibody (2H7), eBioscience

货号：14-0209-82,14-0209-37,14-0209-95

规格：100 μg,2 mg,10 mg

价格：1136,11562,28906

产品类型：流式抗体

品牌：eBioscience

抗原：CD20

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：2H7

荧光染料：其它

抗体类型:	人CD20细胞流式抗体	同型对照：	IgG2b，kappa
浓度：	0.5 mg/mL	用法：	1 µg/test （Flow）;Assay-Dependent（IHC(F））;1:100（ICC（F））

Description:The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.Applications Reported: The 2H7 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections.Applications Tested: The 2H7 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD20 Antibody (14-0209-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD20 FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

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参考文献：

1.PloS oneAn efficient low cost method for gene transfer to T lymphocytes."Published figure using CD20 monoclonal antibody (Product # 14-0209-82) in Flow Cytometry"2.LeukemiaHDAC inhibitors augment cytotoxic activity of rituximab by upregulating CD20 expression on lymphoma cells."14-0209 was used in Flow cytometry/Cell sorting to show that histone deacetylase inhibitors improve rituximabs cytotoxic activity by enhancing the surface expression of CD20 antigen on lymphoma cells."3.Journal of immunology (Baltimore, Md. : 1950)Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation."14-0209 was used in Flow cytometry/Cell sorting to investigate alterations in multifuctional T cell responses during HIV-M. tuberculosis coinfection in an animal model."4.The Journal of experimental medicineCharacterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells."14-0209 was used in Flow cytometry/Cell sorting to investigate a population of human DCs that expresses DNGR-1 and high levels of BDCA3."

技术参数

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