CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE-Cyanine7
货号：25-9942-82
规格：100μg
价格：2844
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
荧光染料：PE-Cy7
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7 |
| 浓度： | 0.2 mg/mL | 用法： | 0.25 μg/test(Flow) |
| 产品详细信息Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.Applications Reported: This 7E.17G9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 7E.17G9 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. |
| 数据 |
| CD278 (ICOS) Antibody (25-9942-82) in FlowStaining of unstimulated (left) or 2-day Con A-stimulated (right) mouse splenocytes with Anti-Mouse CD25 APC (Product # 17-0251-82) and 0.125 µg of Anti-Mouse CD278 (ICOS) PE-Cyanine7. Total viable cells were used for analysis. Quadrant lines were based on staining with 0.125 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82). |
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| 参考文献： |
| 1.Nature communicationsTargeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment."Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry"2.Nature communicationsExtrafollicular CD4+T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease."Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry"3.Nature communicationsRoquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation."Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry" |
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