CD278 (ICOS) Monoclonal Antibody (7E.17G9)
货号：14-9942-85
规格：500 μg
价格：3034
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
荧光染料：其它
| 抗体类型 | 一抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 1 μg/test(Flow) |
| 产品详细信息Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis and for inhibition of ligand binding in functional assays (please use Functional Grade purified, cat. 16-9942).Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and Con A activated (3 day) mouse splenocytes and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. |
| 数据 |
| CD278 (ICOS) Antibody (14-9942-81) in FlowStaining of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b Isotype Control Purified (Product # 14-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD278 (ICOS) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis. |
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| 参考文献： |
| 1.The Journal of experimental medicineInterplay between regulatory T cells and PD-1 in modulating T cell exhaustion and viral control during chronic LCMV infection."14-9942 was used in Flow cytometry/Cell sorting to investigate a role for T reg cells in exhaustion of virus-specific CD8 T cells."2.Cancer immunology, immunotherapy : CIIPoxvirus-based active immunotherapy synergizes with CTLA-4 blockade to increase survival in a murine tumor model by improving the magnitude and quality of cytotoxic T cells."14-9942 was used in Flow cytometry/Cell sorting to help elucidate evidence of enhanced clinical benefit from combining CTLA-4 blockade with poxvirus-based active immunotherapy."3.Nature communicationsExtrafollicular CD4+T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease."Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9942-81) in Flow Cytometry"4.Cancer immunology, immunotherapy : CIIPoxvirus-based active immunotherapy synergizes with CTLA-4 blockade to increase survival in a murine tumor model by improving the magnitude and quality of cytotoxic T cells."14-9942 was used in Flow cytometry/Cell sorting to help elucidate evidence of enhanced clinical benefit from combining CTLA-4 blockade with poxvirus-based active immunotherapy."5.Nature communicationsEffector CD4 T-cell transition to memory requires late cognate interactions that induce autocrine IL-2."14994281 was used in flow cytometry to explore the interaction between IL-2 and IL-7 in CD4 T-cell memory generation" |
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