CD21 Monoclonal Antibody (HB5), PE-Cyanine7, eBioscience™

货号：25-0219-41,25-0219-42

规格：25 tests,100 tests

价格：1217,2921

产品类型：流式抗体

品牌：eBioscience

抗原：CD21

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：HB5

荧光染料：PE-Cy7

抗体类型:	人CD21细胞流式抗体	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7, eBioscience™
浓度：	5 μL/Test	用法：	5μL（0.125 µg）/test (Flow)

Description:The HB5 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein. CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV). CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation.Applications Reported: This HB5 antibody has been reported for usein flow cytometric analysis.Applications Tested: This HB5 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green LaserTarget InformationCD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to follicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD21 functions as receptor for C3d, C3dg and iC3b complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation. Genetic variations in the CD21 gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively, spliced transcript variants encoding different isoforms of CD21 have been found.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD21 Antibody (25-0219-41) in FlowNormal human peripheral blood cells were stained with CD3 Monoclonal Antibody, eFluor 450 (Product # 48-0038-42) and Mouse IgG2a kappa Isotype Control, PE-Cyanine7 (Product # 25-4724-81) (left) or CD21 Monoclonal Antibody, PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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