CD279 (PD-1) Monoclonal Antibody (J43), Biotin
货号：13-9985-82,13-9985-85
规格：100μg,500μg
价格：1649,4331
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：Armenian hamster仓鼠
抗体亚型：IgG
荧光染料：Biotin
| 抗体类型 | 一抗 | 同型对照： | Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin |
| 浓度： | 0.5 mg/mL | 用法： | 0.25 μg/test(Flow) |
| 产品详细信息Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.Applications Reported: The J43 antibody has been reported for use in flow cytometric analysis.Applications Tested: The J43 antibody has been tested by flow cytometric analysis of Con A-simulated mouse splenocytes and mouse PD-1 transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (13-9985-82)eLife 2020 -Figure 3. Establishment of Pmel-1 x SLAMF6 -/- mice as a source of SLAMF6-KO antigen-specific lymphocytes. ( A ) SLAMF6 and Vbeta13 expression in Pmel-1 or Pmel-1 x SLAMF6 -/- splenocytes measured by flow cytometry. ( B ) Percent CD8+, CD4, and CD19 cells in spleens from Pmel-1 or Pmel-1 x SLAMF6 -/- untreated mice. ( C ) Pmel-1, and Pmel-1 x SLAMF6 -/- CD8+ untreated splenocytes were stained with anti-CD44 and anti-CD62L. One representative experiment is shown. ( D ) Percent CD8+ cells in Pmel-1 or Pmel-1 x SLAMF6 -/- splenocytes after 7 days of in vitro activation with gp100 25-33 peptide and IL-2 (30 IU/mL). ( E ) Flow cytometry for activation markers (CD25, CD69, CD137) in Pmel-1 or Pmel-1 x SLAMF6 -/- splenocytes after 3 days of in vitro activation, as in ( D ). Median fluorescence intensity (MFI) is shown. ( F ) Expression of PD-1 in Pmel-1 or Pmel-1 x SLAMF6 -/- CD8+ T cells after 7 days of in vitro activation, as in ( D ). Median fluorescence intensity (MFI) is shown. ( G, H ) After 7 days of activation, Pmel-1 and Pmel-1 x SLAMF6 -/- CD8+ T cells were stained with anti-CD44 and anti-CD62L. CD8+ subpopulations were defined for each mouse strain. ( G ) One representative experiment and ( H ) summary of subpopulations identified by flow cytometry in five experiments is shown. EM, effector memory, CM, central memory. Student t-test. *, p<0.05, **, p<0.01, ***, p<0.001. Figure 3--figure supplement 1. Characterization of Pmel-1 x SLAMF6 -/- mice. ( A ) Immunohistochemistry |
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| 参考文献： |
| 1.Journal of CancerPD-1 Blockade Overcomes Adaptive Immune Resistance in Treatment with Anchored-GM-CSF Bladder Cancer Cells Vaccine."Published figure using CD279 (PD-1) monoclonal antibody (Product # 13-9985-82) in Immunohistochemistry"2.Gene therapyEffects of irradiating adult mdx mice before full-length dystrophin cDNA transfer on host anti-dystrophin immunity."13-9985 was used in Immunohistochemistry to demonstrate that dystrophin gene transfer induces anti-dystrophin immunity that are diminished by temporal removal of host immune cells."3.The Journal of experimental medicineThe programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.AuthorsAnsari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH4.OncoimmunologyThe stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas."Published figure using CD279 (PD-1) monoclonal antibody (Product # 13-9985-82) in Flow Cytometry"5.OncoimmunologyGrowth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression."Published figure using CD279 (PD-1) monoclonal antibody (Product # 13-9985-82) in Flow Cytometry" |
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