CD80 (B7-1) Monoclonal Antibody (2D10.4), Biotin, eBioscience

货号：13-0809-82

规格：100 µg

价格：1888

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：2D10.4

荧光染料：Biotin

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	1.0µg/test（Flow）

产品详细信息Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 2D10.4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (13-0809-82)OncoTargets and therapy 2015 -Published figure using CD80 (B7-1) monoclonal antibody (Product # 13-0809-82)

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献：

1.Oncology lettersPhotodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70."Published figure using CD80 (B7-1) monoclonal antibody (Product # 13-0809-82) in Flow Cytometry"2.NutrientsAstragalus polysaccharides(PG2) Enhances the M1 Polarization of Macrophages, Functional Maturation of Dendritic Cells, and T Cell-Mediated Anticancer Immune Responses in Patients with Lung Cancer."Published figure using CD80 (B7-1) monoclonal antibody (Product # 13-0809-82) in Flow Cytometry"3.Infection and immunityHumanized mice, a new model to study the influence of drug treatment on neonatal sepsis."13-0809 was used in Flow cytometry/Cell sorting to investigate the use of humanised mice as a model of GBS-induced sepsis."AuthorsErnst W,Zimara N,Hanses F,Männel DN,Seelbach-Göbel B,Wege AK4.The Journal of clinical investigationRenal allograft rejection is prevented by adoptive transfer of anergic T cells in nonhuman primates.AuthorsBashuda H,Kimikawa M,Seino K,Kato Y,Ono F,Shimizu A,Yagita H,Teraoka S,Okumura K

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-27751.htm