CD80 (B7-1) Monoclonal Antibody (2D10.4), FITC, eBioscience 7个已发表图片

货号：11-0809-42,11-0809-41

规格：100 Tests,25 Tests

价格：1888,838

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：2D10.4

荧光染料：FITC

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience
浓度：	5 µL/Test	用法：	5 µL (1 µg)/test（Flow）

产品详细信息Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (11-0809-42) in FlowStaining of the Daudi cell line with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD80 (B7-1) FITC (purple histogram). Total viable cells were used for analysis.

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参考文献：

1. Experimental and therapeutic medicineMALP-2, an agonist of TLR6, promotes the immune status without affecting the differentiation capacity of umbilical cord mesenchymal stem cells."11-0809 was used in Flow cytometry/Cell sorting to assess the role of TLR6 in the immune status of umbilical cord mesenchymal stem cells, by stimulation with the TLR6 agonist MALP-2."AuthorsWu X,Xu L,Shen Y,Yu N,Zhang Y,Guo TYear2017SpeciesHumanDilution1:200 2. Journal of immunology (Baltimore, Md. : 1950)Antigen-Presenting Human γδ T Cells Promote Intestinal CD4+T Cell Expression of IL-22 and Mucosal Release of Calprotectin.AuthorsTyler CJ,McCarthy NE,Lindsay JO,Stagg AJ,Moser B,Eberl MYear2017SpeciesHuman 3. BMC cancerCD80 down-regulation is associated to aberrant DNA methylation in non-inflammatory colon carcinogenesis."11-0809 was used in Flow cytometry/Cell sorting to indicate that the failure of immune surveillance mechanisms in non-inflammatory colon carcinogenesis may affect CD80 expression."AuthorsScarpa M,Scarpa M,Castagliuolo I,Erroi F,Basato S,Brun P,Angriman I,Castoro CYear2016SpeciesHuman 4. International journal of molecular medicineThe role of Toll-like receptor 3 and 4 in regulating the function of mesenchymal stem cells isolated from umbilical cord."11-0809 was used in Western Blotting to examine the role of the TLR3 and TLR4 pathways in modulating the immune properties of human umbilical cord-derived MSCs."AuthorsZhang L,Liu D,Pu D,Wang Y,Li L,He Y,Li Y,Li L,Qiu Z,Zhao S,Li WYear2015SpeciesHuman 5. Journal of immunology (Baltimore, Md. : 1950)HIV-1 and HIV-2 differentially mature plasmacytoid dendritic cells into IFN-producing cells or APCs."11-0809 was used in Flow cytometry/Cell sorting to study the factors causing the observed differences in pathogenicity between HIV-1 and HIV-2."AuthorsRoyle CM,Graham DR,Sharma S,Fuchs D,Boasso A

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