CD279 (PD-1) Monoclonal Antibody (J43)
货号：14-9985-82,14-9985-85
规格：100μg,500μg
价格：1752,3274
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：Armenian hamster仓鼠
抗体亚型：IgG
荧光染料：其它
| 抗体类型 | 一抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 0.5 μg/test (Flow)； |
| 产品详细信息Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.Applications Reported: The J43 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported for use in in vitro functional assays. (Please use Functional Grade purified J43, cat. 16-9985, in functional assays.).Applications Tested: The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cells and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (14-9985-81) in FlowStaining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 µg of Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1.Journal of CancerPD-1 Blockade Overcomes Adaptive Immune Resistance in Treatment with Anchored-GM-CSF Bladder Cancer Cells Vaccine."Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9985-82) in Immunohistochemistry"2.The Journal of experimental medicineThe programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.3.OncoimmunologyThe stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas."Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9985-82) in Flow Cytometry"4.OncoimmunologyGrowth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression."Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9985-82) in Flow Cytometry"5.Journal of CancerPD-1 Blockade Overcomes Adaptive Immune Resistance in Treatment with Anchored-GM-CSF Bladder Cancer Cells Vaccine."Published figure using CD279 (PD-1) monoclonal antibody (Product # 14-9985-82) in Immunohistochemistry" |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-27756.htm
QQ:1749072012