CD23 Monoclonal Antibody (EBVCS2), APC-eFluor 780, eBioscience™

货号：47-0238-42

规格：100 Tests

价格：3202

产品类型：流式抗体

品牌：eBioscience

抗原：CD23

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号： EBVCS2

荧光染料：APC-eFluor 780

类型:	人B细胞流抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor 780, eBioscience™
浓度：	5 µL/Test	用法：	5 μL (0.125 μg)/test(Flow)

产品详细信息Description: The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23.Applications Reported: This EBVCS2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This EBVCS2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood andtonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD23 Antibody (47-0238-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD23 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.ImmunologyInterleukin-25 fails to activate STAT6 and induce alternatively activated macrophages."Published figure using CD23 monoclonal antibody (Product # 47-0238-42) in Flow Cytometry"

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