CD279 (PD-1) Monoclonal Antibody (RMP1-30), APC
货号：13-9981-80,13-9981-82
规格：25μg,100μg
价格：0,1649
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：APC
| 抗体类型 | 一抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), APC |
| 浓度： | 0.2 mg/mL | 用法： | 1 μg/test (Flow)； |
| 产品详细信息Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligandsand their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.Applications Reported: This RMP1-30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RMP1-30 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (17-9981-80) in FlowStaining of unstimulated (orange) or ConA-stimulated (purple) splenocytes with 0.5 µg of Anti-Mouse CD279 (PD-1) APC. Total viable cells were used for analysis. |
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| 参考文献： |
| 1.The Journal of experimental medicineCreatine uptake regulates CD8 T cell antitumor immunity."Published figure using CD279 (PD-1) monoclonal antibody (Product # 17-9981-82) in Flow Cytometry"2.Nature communicationsInterdependent IL-7 and IFN-γ signalling in T-cell controls tumour eradication by combined α-CTLA-4+α-PD-1 therapy."17-9981 was used in Flow cytometry/Cell sorting to study how tumour eradication can be controlled by interdependent IL-7 and IFN-γ signalling in T-cells."3.EBioMedicineAdjuvant-enhanced CD4 T Cell Responses are Critical to Durable Vaccine Immunity."17-9981 was used in Flow cytometry/Cell sorting to demonstrate that serum and CD4 T-cells play a critical role in conferring protection within effective vaccination regimens."4.Journal of virologyCD4 T cells promote CD8 T cell immunity at the priming and effector site during viral encephalitis."17-9981 was used in Flow cytometry/Cell sorting to demonstrate that CD4 T cells play critical roles in both enhancing peripheral activation of CD8 T cells and prolonging their antiviral function within the CNS." |
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