| 产品介绍 | | |
| Rabbit anti Human PDGF-B antibodyrecognizes platelet-derived growth factor subunit B, also known as PDGF subunit B, becaplermin, platelet-derived growth factor 2, platelet-derived growth factor beta polypeptide or proto-oncogene c-Sis.The protein encoded by the PDGFB gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGFB product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimer chains are connected by disulfide bonds.Mutations in PDGFB are associated withmeningiomaand Basal ganglia calcification, idiopathic, 5 (IBGC5), an autosomal dominant disorder characterized by progressive neurologic symptoms associated with brain calcifications (Kelleret al.2013). Reciprocal translocations between chromosomes 22 and 17, at sites where PDGFB and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans (DFSB) resulting from unregulated expression of growth factor (Kiuru-Kuhlefeltet al.2001). Two alternatively spliced transcript variants encoding different isoforms have been identified for PDGFB (provided by RefSeq, Feb 2013).Rabbit anti Human PDGF-B antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Goat polyclonal antibody purified by affinity chromatography. |
| Buffer Solution | TRIS buffered saline. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)0.5% Bovine Serum Albumin. |
| Immunogen | Synthetic peptide directed towards the N terminal region of human PDGF-B |
| Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store undiluted at -20°C, avoiding repeated freeze thaw cycles |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
QQ:1749072012