| 产品介绍 | | |
| Rabbit anti Human MIP-1 beta antibodyrecognizes human MIP-1 beta, otherwise known as CCL4, a 69 amino acid member of the CC chemokine family, shown to signal through the chemokine receptors CCR1, CCR2 and CCR5.MIP-1 beta is involved in host defence against invading pathogens, acting as a regulator of activation and trafficking of inflammatory cells, such as monocytes, B cells, NK cells, neutrophils, dendritic cells and CD4+ lymphocytes.The secretion of MIP-1 beta by CD8+ lymphocytes has been shown to act as a major HIV-suppressive factor (HIV-SF) through binding to CCR5, identified as a coreceptor for HIV-1 target cell entry. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
| Reconstitution | Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide. |
| Antiserum Preparation | Antisera to human MIP-1 beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | None present |
| Carrier Free | Yes |
Immunogen | Recombinant human MIP-1 beta. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | 0.5ug/ml |
| Functional Assays | √ | 1.75 | 3.0ug/ml |
| Immunohistology - Paraffin 1 | √ | | |
Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
References for MIP-1 Beta antibody- Cocchi, F.et al.(1995) Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells.Science. 270 (5243): 1811-5.
Further Reading- Menten, P.et al.(2002) Macrophage inflammatory protein-1.Cytokine Growth Factor Rev. 13 (6): 455-81.
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