Rabbit anti Human U2AF2 antibody
货号:VPA00241
规格:100μl
价格:2916
产品类型:免疫组化一抗
品牌:Bio-Rad
抗原:U2AF2
物种:人
宿主:兔
抗体亚型:IgG
荧光染料:Purified
产品介绍 | | |
Rabbit anti Human U2AF2 antibodyrecognizes the splicing factor U2AF 65 kDa subunit, also known as U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 snRNP auxiliary factor large subunit and hU2AF65.Splicing factor U2AF is comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 gene encodes the large 65 kDa subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for U2AF2, but the full-length natures of only two have been determined to date (provided by RefSeq, Jul 2008).Rabbit anti Human U2AF2 antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Rabbit polyclonal antibody purified by affinity chromatography. |
Buffer Solution | Phosphate buffered saline. |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3). |
Immunogen | KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human U2AF2 |
Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch. |
存储条件 | | |
Store undiluted at -20°C, avoiding repeated freeze thaw cycles. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 1/1000 | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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