Mouse anti Human 14-3-3 epsilon antibody
货号：VMA00525
规格：100μl
价格：2916
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：14-3-3 epsilon
物种：人
宿主：小鼠
抗体亚型：IgG2a
克隆号：AB01/1G9
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human 14-3-3 epsilon antibodyrecognizes the 14-3-3 protein epsilon, also known as epididymis luminal protein 2, mitochondrial import stimulation factor L subunit, protein kinase C inhibitor protein-1, or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, or YWHAE.YWHAE gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for YWHAE (provided by RefSeq, Aug 2008).Mouse anti Human 14-3-3 epsilon antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3). |
| Immunogen | E. coli-derived recombinant human 14-3-3 epsilon (amino acids 1 - 255) |
| Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch. |
| 存储条件 | | |
| Store undiluted at -20°C, avoiding repeated freeze thaw cycles. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/1000 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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技术参数 产品应用 wb
产品类型 单克隆抗体
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