| 产品介绍 | | |
| Mouse anti Human CD16 antibody, clone LNK16recognizes CD16, a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes.CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by ion exchange chromatography |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
Immunogen | Normal human peripheral blood granulocytes. |
Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| Max Ex/Em | 490/525 |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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Description | Product Code | Applications | Pack Size |
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| 文献 |
| 1. Tamm, A. & Schmidt, R.E. (1996) The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.J Immunol. 157 (4): 1576-81.2. Fleit, H.B.et al.(1982) Human neutrophil Fc gamma receptor distribution and structure.Proc Natl Acad Sci U S A. 79 (10): 3275-9.3. van de Winkel, J.G. & Capel, P.J. (1993) Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.Immunol Today. 14 (5): 215-21.4. Pillay, J.et al.(2012) A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1.J Clin Invest. 122: 327-36.5. Morris, D.L.et al.(2010) Evidence for both copy number and allelic (NA1/NA2) risk at the FCGR3B locus in systemic lupus erythematosus.Eur J Hum Genet. 18 (9): 1027-31.6. Kamp, V.M.et al.(2012) Human suppressive neutrophils CD16bright/CD62Ldim exhibit decreased adhesion.J Leukoc Biol. 92 (5): 1011-20.7. Hanna, J.et al.(2003) CXCL12 expression by invasive trophoblasts induces the specific migration of CD16- human natural killer cells.Blood. 102:1569-77.8. Moalli, F.et al.(2010) Role of complement and Fc{gamma} receptors in the protective activity of the long pentraxin PTX3 against Aspergillus fumigatus.Blood. 116: 5170-80. |
QQ:1749072012