| 产品介绍 | | |
| Goat F(ab′)2anti Human IgG antibodyrecognizes human IgG and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses.Goat F(ab′)2anti Human IgG antibody does not cross-react with Goat IgG F(c) or mouse, rat, bovine and horse serum proteins, as determined by immunoelectrophoresis.Goat F(ab′)2anti Human IgG antibody has been used for the detection of the antibody reponse to an endogenous retrovirus GAG matrix in patients with rheumatoid arthritis by ELISA (Nelsonet al.2014). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | F(ab')2fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Antiserum Preparation | Antisera to human IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2fragments were prepared by pepsin digestion. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Approx. Protein Concentrations | IgG concentration 0.7 mg/ml |
| Immunogen | Human IgG. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/50 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Nelson, P.N.et al.(2014) Rheumatoid Arthritis is Associated with IgG Antibodies to Human Endogenous Retrovirus Gag Matrix: A Potential Pathogenic Mechanism of Disease?J Rheumatol. 41: 1952-60.2.Trela, M.et al.(2019) Citrullination facilitates cross-reactivity of rheumatoid factor with non-IgG1 Fc epitopes in rheumatoid arthritis.Sci Rep. 9 (1): 12068. |
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