| 产品介绍 | | |
| Brain-derived neurotrophic factor (BDNF) is a potent neurotrophin and member of the NGF-beta family, secreted from dense core vesicles, which promotes the growth, differentiation and survival of neurons within, or directly connected to, the central nervous system.BDNF signals through binding to the high affinity receptor trkB, and also binds to the low affinity neurotrophin receptor LNGFR. BDNF is involved in the modulation of short-term synaptic function and in the formation of long-term memory, and reduced levels of BDNF are seen in patients with Alzheimer's and Huntington disease (Girotaet al.2022). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Preparation | Purified recombinant BDNF expressed inE.coli |
| Reconstitution | Reconstitute with 100 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Activity | Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50for this effect is 0.5-1.0 μg/ml. |
| Preservative Stabilisers | None present |
| Approx. Protein Concentrations | 1.0 mg/ml after reconstitution |
| Protein Molecular Weight | 27.0 kDa (119 amino acid homodimer) |
| Purity | >98% by SDS PAGE and HPLC analysis |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| Prior to reconstitution store at -20℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Functional Assays | √ | | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
QQ:1749072012