产品介绍 | | |
Recombinant Human CD154is anE.coliderived protein corresponding to human CD154, also known as CD40 ligand, CD40L or TRAP. CD154 is a membrane protein expressed by activated T cells. Binding of CD154 to its ligand (CD40) on B cells induces B cell proliferation and immunoglobulin class switching.This preparation of Recombinant Human CD154 comprises the receptor binding TNF-like domain of human CD154. |
产品详情 | | |
Target Species | Human |
Product Form | Purified recombinant protein - lyophilized |
Reconstitution | Reconstitute with 10ul 5-10mM sodium phosphate pH7.5. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
Preservative Stabilisers | None present |
Carrier Free | Yes |
Purity | >98% by SDS PAGE and HPLC sequence analysis |
Protein Molecular Weight | 16.3kDa (149 amino acid sequence) |
Approx. Protein Concentrations | 0.5 mg/ml after reconstitution. |
Regulatory | For research purposes only |
Guarantee | 3 months from date of reconstitution |
存储条件 | | |
Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Functional Assays | √ | | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |