| 产品介绍 | | |
| Mouse anti Human CD151 antibody, clone 11G5arecognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen (Crewet al.2004).It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (Yauch.et al.1998). |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1 ml distilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin 5% Sucrose |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:PE | MCA928PE | Flow | 100 Tests |
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| 文献 |
| 1. Spoden, G.et al.(2008) Clathrin- and caveolin-independent entry of human papillomavirus type 16--involvement of tetraspanin-enriched microdomains (TEMs).PLoS One. 3: e3313.2. Huang, X.Y.et al.(2010) Overexpression of CD151 as an adverse marker for intrahepatic cholangiocarcinoma patients.Cancer. 116: 5440-51.3. Ke, A.W.et al.(2009) Role of overexpression of CD151 and/or c-Met in predicting prognosis of hepatocellular carcinoma.Hepatology. 49: 491-503.4. Devbhandari, R.P.et al.(2011) Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma.PLoS One. 6: e24901.5. Shi, G.M.et al.(2010) CD151 modulates expression of matrix metalloproteinase 9 and promotes neoangiogenesis and progression of hepatocellular carcinoma.Hepatology. 52: 183-96.6. Nishiuchi, R.et al.(2005) Potentiation of the ligand-binding activity of integrin alpha3beta1 via association with tetraspanin CD151.Proc Natl Acad Sci U S A.102: 1939-44.7. Qiao, Y.et al.(2017) CD151, a laminin receptor showing increased expression in asthmatic patients, contributes to airway hyperresponsiveness through calcium signaling.J Allergy Clin Immunol. 139 (1): 82-92.e5.8. Hochdorfer, D.et al.(2016) Tetraspanin CD151 Promotes Initial Events in Human Cytomegalovirus Infection.J Virol. 90 (14): 6430-42.9. Wadkin, J.C.R.et al.(2017) CD151 supports VCAM-1-mediated lymphocyte adhesion to liver endothelium and is upregulated in chronic liver disease and hepatocellular carcinoma.Am J Physiol Gastrointest Liver Physiol. 313 (2): G138-G149.10. Burkard, C.et al.(2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.PLoS Pathog. 13 (2): e1006206. |
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