Mouse anti Human CD53 antibody, clone MEM-53
货号：MCA723F
规格：100 Tests
价格：2907
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD53
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：MEM-53
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Human CD53 antibody, clone MEM-53recognizes the human CD53 cell surface antigen, also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by ion exchange chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
| 文献 |
| 1.Dunlock, V.E.et al.(2019) Dynamic regulation of CD45 by tetraspanin CD53bioRxiv Nov 29 [Epub ahead of print].2.Bazil, V.et al.(1989) Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.Folia Biol (Praha). 35 (5): 289-97.3.Angelisová Pet al.(1990) The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens.Immunogenetics. 32 (4): 281-5.4.Mollinedo, F.et al.(1997) Recurrent infectious diseases in human CD53 deficiency.Clin Diagn Lab Immunol. 4: 229-31. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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