Mouse anti Pig CD8 beta chain, clone PPT23
货号：MCA5954F
规格：0.1 mg
价格：3766
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD8 beta
物种：pig
宿主：小鼠
抗体亚型：IgG1
克隆号：PPT23
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hiand as such defines this antibody as a specific marker of porcine α/β T cells, in thymic tissue both CD8loand CD8hicells are recognized (Yang & Parkhouse 1997). Mouse anti Pig CD8 beta chain, clone PPT23 recognizes a different epitope on the CD8 beta chain to clone PPT22. |
| 产品详情 | | |
| Target Species | Pig |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Porcine thymus membrane lysate |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Fusion Partners | Lymph node cells from immunized BALB/c mice were fused with cells of the NSO myeloma cell line |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Yang, H. & Parkhouse, R.M. (1997) Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets.Immunology. 92 (1): 45-52.2.Mair, K.H.et al.(2015) Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS®MLV.Vet Microbiol. 176 (3-4): 352-7.3.Sinkora, M.et al.(2007) Two groups of porcine TCRgammadelta+ thymocytes behave and diverge differently.J Immunol. 178 (2): 711-9. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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