| 产品介绍 | | |
| Mouse anti Bovine CD21 monoclonal antibody, clone CC51recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiplesushidomains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessenset al.1990). CD21 may be expressed on B-cells as either a long or a short form (Pringleet al.2012).Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkoraet. al.2013). In addition to clone CC51, clone CC21 (MCA1424GA) which has been demonstrated to recognise CD21 in a range of ruminant and other species is also available from Bio-Rad. |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG conjugated to Alexa Fluor 647 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Bovine (Friesian cattle) mesenteric lymph node cells |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®647 | 650 | 665 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Sinkora, M.et al.(2014) The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2.Vet Res. 45: 91.2.Sinkora, Met al.(2013) Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs.Dev Comp Immunol. 39: 409-18.3.Tenaya I.W.et al.(2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.Vet Immunol Immunopathol. 149: 167-76.4.Denham S.et al.(1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.Vet Immunol Immunopathol. 43: 259-67. |
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