Mouse anti Sheep CD14 monoclonal antibody, clone VPM66
货号：MCA5939GA
规格：0.1 mg
价格：2185
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD14
物种：绵羊
宿主：小鼠
抗体亚型：IgG1
克隆号：VPM66
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Sheep CD14 monoclonal antibody, clone VPM66recognises ovine CD14, a GPI-anchored ~55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes (Hopkinset. al.1996).CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein. The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.Clone VPM66 has been shown to recognise CD14 in other domestic ruminant species including Water Buffalo (Bubalus bubalis) (Vimoset. al.1996) and domestic cattle (Hopkinset. al.1996). In addition to clone VPM66, clonesVPM65andVPM67are also available. |
| 产品详情 | | |
| Target Species | Sheep |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Ovine macrophages. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/25 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | F | 0.5mg |
| 文献 |
| 1.Hopkins, J. & Gupta, V.K. (1996) Identification of three myeloid-specific differentiation antigens in sheep.Vet Immunol Immunopathol. 52 (4): 329-39.2.Akesson, C.P.et al.(2008) Phenotypic characterisation of intestinal dendritic cells in sheep.Dev Comp Immunol. 32 (7): 837-49.3.Gupta, V.K.et al.(1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.Vet Immunol Immunopathol. 51 (1-2): 89-99.4.Vilmos, P.et al.(1996) Phylogenetically conserved epitopes of leukocyte antigens.Vet Immunol Immunopathol. 52 (4): 415-26. |
技术参数 产品应用 Flow；IP
产品类型 Monoclonal Antibody
推荐用于 Flow；IP
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