CD335 (NKp46) Monoclonal Antibody (29A1.4), PE-eFluor 610
货号：61-3351-82
规格：100μg
价格：2947
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：PE-eFluor 610
| 抗体类型 | 单抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610 |
| 浓度： | 0.2 mg/mL | 用法： | 1 μg/test (Flow)； |
| 产品详细信息Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR). |
| 数据 |
| CD335 (NKp46) Antibody (61-3351-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) and 0.5 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or 0.5 µg of Anti-Mouse CD335 (NKp46) PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Nature communicationsNK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation."Published figure using CD335 (NKp46) monoclonal antibody (Product # 61-3351-82) in Flow Cytometry"2.Cellular and molecular immunologyThe breast tumor microenvironment alters the phenotype and function of natural killer cells."Published figure using CD335 (NKp46) monoclonal antibody (Product # 61-3351-82) in Flow Cytometry"3.Wellcome open researchConventional NK cells and ILC1 are partially ablated in the livers of Ncr1iCreTbx21fl/flmice."Published figure using CD335 (NKp46) monoclonal antibody (Product # 61-3351-82) in Flow Cytometry"4.Cellular and molecular immunologyThe breast tumor microenvironment alters the phenotype and function of natural killer cells."Published figure using CD335 (NKp46) monoclonal antibody (Product # 61-3351-82) in Flow Cytometry"5.Nature immunologyIL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection with Citrobacter rodentium.AuthorsOta N,Wong K,Valdez PA,Zheng Y,Crellin NK,Diehl L,Ouyang W |
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